Integrated single-molecule long-read sequencing and Illumina sequencing reveal the resistance mechanism of Psathyrostachys huashanica in response to barley yellow dwarf virus-GAV

Table 1 Summary of reads of insert (ROIs) classification, the size fraction of F01 and G01 libraries were 1–5 kb, and the size fraction of H01 library was 4.5-10 kb

Sample	Library	Reads of insert	Five prime reads	Three prime reads	Poly-A reads	Full-length non-chimeric reads	Full-length non-chimeric read length (bp)
Psathyrostachys huashanica	F01	565,467	301,093 (53.25%)	316,921 (56.05%)	292,646 (51.75%)	194,333 (34.37%)	451.5
Psathyrostachys huashanica	G01	575,061	345,129 (60.02%)	360,354 (62.66%)	333,960 (58.07%)	232,059 (40.35%)	457.5
Psathyrostachys huashanica	H01	547,831	267,902 (48.9%)	269,092 (49.12%)	139,934 (25.54%)	80,712 (14.73%)	2723